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Related Experiment Videos

A human NDP-kinase B specifically binds single-stranded poly-pyrimidine sequences

M Hildebrandt1, M L Lacombe, S Mesnildrey

  • 1Unité de Biochimie Cellulaire, CNRS-URA 1129, Institut Pasteur, Paris, France.

Nucleic Acids Research
|October 11, 1995
PubMed
Summary

Nucleoside diphosphate kinase B (NDPK-B) binds single-stranded DNA, challenging its role as a transcription factor. This enzyme interacts with various DNA and RNA sequences, suggesting diverse cellular functions beyond transcription regulation.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • A DNA-binding protein, PUF, was identified binding to the human c-myc promoter.
  • This protein was unexpectedly identified as nucleoside diphosphate kinase B (NDPK-B), also known as nm23-H2.

Purpose of the Study:

  • To investigate the DNA-binding properties of NDPK-B.
  • To determine if NDPK-B functions as a transcription factor.

Main Methods:

  • Electrophoretic mobility shift assay (EMSA) using recombinant NDP kinases.
  • Analysis of binding to various single-stranded and double-stranded DNA and RNA sequences.

Main Results:

  • NDPK-B exhibits DNA-binding properties, predominantly with human isoform B.

Related Experiment Videos

  • Binding occurs to single-stranded DNA, not double-stranded DNA, independent of ATP.
  • NDPK-B binds to diverse poly-pyrimidine rich sequences, including DNA and RNA.

    • Conclusions:

    • NDPK-B's DNA-binding characteristics differ from typical transcription factors.
    • The findings suggest broader roles for NDP kinase beyond transcriptional regulation.