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Related Experiment Videos

CD27-CD70 interactions regulate B-cell activation by T cells

T Kobata1, S Jacquot, S Kozlowski

  • 1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 21, 1995
PubMed
Summary
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Interactions between CD27 and CD70 on T cells regulate B-cell activation and immunoglobulin production. CD27 inhibits, while CD70 enhances, B-cell responses in a T-cell dependent system.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD27, a tumor necrosis factor (TNF) receptor family member, binds CD70, a TNF family ligand.
  • This interaction induces T-cell costimulation and B-cell activation.
  • CD27 is found on resting T and B cells, while CD70 is on activated T and B cells.

Purpose of the Study:

  • To investigate the impact of CD27 and CD70 interactions on human B-cell IgG production and proliferation.
  • To elucidate the role of T-cell subsets expressing CD27 and CD70 in regulating B-cell responses.

Main Methods:

  • Utilized transfected murine pre-B-cell lines expressing human CD27 or CD70.
  • Examined effects on pokeweed mitogen-driven (T-cell dependent) and Staphylococcus aureus/interleukin 2-driven (T-cell independent) B-cell activation systems.
  • Assessed IgG production and B-cell proliferation with and without anti-CD27/anti-CD70 monoclonal antibodies.

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Main Results:

  • CD27-transfected cells inhibited IgG production in T-cell dependent systems, while CD70-transfected cells enhanced it.
  • These effects were blocked by respective monoclonal antibodies.
  • In T-cell independent systems, CD70 enhanced IgG production and proliferation, which was inhibited by CD27-transfected cells.

Conclusions:

  • T-cell interactions involving CD27 and CD70 are crucial regulators of B-cell activation.
  • CD27 and CD70 play distinct, opposing roles in modulating B-cell immunoglobulin synthesis and proliferation.
  • The context of T-cell dependency significantly influences the outcome of CD27/CD70 signaling on B cells.