Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An ecdysiostatin from flies

Y J Hua1, J Koolman

  • 1Institute of Physiological Chemistry, Philipps-University, Marburg, Germany.

Regulatory Peptides
|June 27, 1995
PubMed
Summary
This summary is machine-generated.

Researchers developed a bioassay to find ecdysiostatins in blowfly larvae, identifying a hexapeptide that inhibits ecdysone biosynthesis by increasing cAMP levels, acting as a prothoracicostatic hormone.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prospective association study of sleep status and risk of coronary heart disease in adults in Suzhou].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

[A prospective study on association between sleep duration and the risk of chronic obstructive pulmonary disease in adults in Suzhou].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

[Three-dimensional finite element study on the effects of maxillary anterior two-step retraction and en-masse retraction with clear aligners].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2023
Same author

[Incidence of chronic obstructive pulmonary disease and risk factors in the Suzhou cohort].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2023
Same author

Potentially improved response of COVID-19 vaccinated nasopharyngeal cancer patients to combination therapy with anti-PD-1 blockade and chemotherapy.

Annals of oncology : official journal of the European Society for Medical Oncology·2022
Same author

[Incidence of gastric cancer and risk factors in Suzhou cohort].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2022
Same journal

The merger of Regulatory Peptides and Peptides.

Regulatory peptides·2014
Same journal

WITHDRAWN: Effects of centrally-injected glucagon-like peptide-2 on gastric mucosal blood flow in rats; possible mechanisms.

Regulatory peptides·2014
Same journal

Hypothalamic-pituitary thyroid axis alterations in female mice with deletion of the neuromedin B receptor gene.

Regulatory peptides·2014
Same journal

Resistin induces lipolysis and suppresses adiponectin secretion in cultured human visceral adipose tissue.

Regulatory peptides·2014
Same journal

Insulin-like growth factor-1 inhibits colonic smooth muscle cell apoptosis in diabetic rats with colonic dysmotility.

Regulatory peptides·2014
Same journal

Prior regular exercise reverses the decreased effects of sleep deprivation on brain-derived neurotrophic factor levels in the hippocampus of ovariectomized female rats.

Regulatory peptides·2014
See all related articles

Area of Science:

  • Insect endocrinology
  • Molecular biology
  • Biochemistry

Background:

  • Ecdysteroids are critical insect hormones regulating molting and metamorphosis.
  • The biosynthesis and regulation of ecdysteroids are complex and not fully understood.
  • Previous research suggested the existence of factors inhibiting ecdysone production in insect larvae.

Purpose of the Study:

  • To develop a bioassay for identifying compounds with ecdysiostatic activity.
  • To confirm the presence of an ecdysiostatin in blowfly larvae.
  • To elucidate the mechanism of action of the identified ecdysiostatin.

Main Methods:

  • Development of a bioassay for ecdysiostatic compounds.
  • High-performance liquid chromatography (HPLC) for factor isolation.

Related Experiment Videos

  • Testing the specificity and activity of the isolated hexapeptide.
  • Measurement of intracellular cyclic adenosine monophosphate (cAMP) levels in ring glands.

    • Main Results:

    • A novel bioassay successfully identified ecdysiostatic activity in blowfly larvae.
    • A specific hexapeptide, similar to Neb-TMOF (trypsin modulating oostatic factor), was isolated.
    • This hexapeptide inhibits ecdysone biosynthesis, not secretion, and increases cAMP levels.
    • Elevating intracellular cAMP mimicked the ecdysiostatic effect, suggesting a signaling pathway.

    Conclusions:

    • The identified hexapeptide functions as a prothoracicostatic hormone (PTSH).
    • PTSH controls ecdysone biosynthesis in fly larvae via a cAMP-dependent pathway.
    • This discovery provides new insights into insect steroidogenesis regulation.