An ecdysiostatin from flies

A bioassay for compounds with ecdysiostatic activity ('ecdysiostatins') was developed in order to prove the existence of an ecdysiostatin in blowfly larvae. The factor eluted by HPLC like Neb-TMOF (trypsin modulating oostatic factor), a hexapeptide that inhibits ecdysone biosynthesis. The ecdysiostatic activity of Neb-TMOF is specific, related peptides were less active or inactive. TMOF inhibits ecdysone biosynthesis rather than its secretion. It increases the cAMP level of ring glands. Effectors that elevate the intracellular cAMP can mimic the inhibitory effect of the ecdysiostatin. This suggests that cAMP may control steroidogenesis in fly larvae by shutting ecdysone biosynthesis. Thus, the hexapeptide acts as a prothoracicostatic hormone (PTSH) that controls ecdysone biosynthesis.