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Updated: Aug 11, 2026
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
Published on: April 3, 2011
[Characterization of mutation of the P53 gene in lymphomas]
B Martínez-Delgado1, M Robledo, E Arranz
1Departamento de Genética, Fundación Jiménez Díaz, Madrid.
Purpose:
To analyze P53 mutations in a series of NHL and to determine its implication in the inactivation of the tumor suppressor function.
Material And Methods:
We analyzed 18 lymphomas by SSCP (exons 5 to 9) and subsequent sequencing technics to detect and characterize mutations on this gene. Loss of heterozigosity (LOH) was also studied with a VNTR near the p53 gene and with a intragenic microsatellite, comparing tumor and normal tissue.
Results:
We found altered bands by SSCP in 5 cases, 4 of them have been described by sequencing analysis. One case was a polymorphism and the others were missense mutations. All mutations appeared in high grade lymphomas. Only one case showed LOH in both VNTR and microsatellite.
Conclusions:
This results suggest that p53 mutations may be associated with more malignant lymphomas.
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