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A method for producing microbe-free Amyloodinium ocellatum (Brown) with Percoll
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Veterinary Parasitology
|September 1, 1995
Summary
Researchers developed a method to collect microbe-free Amyloodinium ocellatum from infected fish. This technique uses antibiotic washes and Percoll density gradient purification for sterile dinospore collection.
Area of Science:
- Ichthyopathology
- Marine Biology
- Parasitology
Background:
- Amyloodinium ocellatum is a significant parasite affecting marine fish, including red drum (Sciaenops ocellatus).
- Accurate study of this parasite requires pure, uncontaminated samples for research and diagnostics.
Purpose of the Study:
- To describe a method for obtaining microbe-free Amyloodinium ocellatum.
- To ensure the purity of parasite samples for further research.
Main Methods:
- Trophonts were isolated from infected red drum using osmotic shock.
- Preparations were washed with antibiotic-containing saltwater and purified using Percoll density gradient.
- Purified tomonts were incubated in antibiotic-containing saltwater to induce excystment of dinospores.
Main Results:
- The described method effectively reduced surface contamination on trophonts.
- Percoll density gradient purification yielded a tomont preparation free of external microbial contamination.
- Sterile, infective dinospores were successfully obtained after 72 hours of incubation.
Conclusions:
- A reliable method for collecting sterile Amyloodinium ocellatum dinospores has been established.
- This technique is crucial for future research on fish parasites and disease control.