Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

One-electron oxidations by peroxidases

H B Dunford1

  • 1Department of Chemistry, University of Alberta, Edmonton, Canada.

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of rates and equilibria for keto-enol tautomerism of aldehydes using horseradish peroxidase compound I.

Journal of the American Chemical Society·2012
Same author

How do enzymes work? Effect of electron circuits on transition state acid dissociation constants.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2001
Same author

Mechanism of reaction of myeloperoxidase with hydrogen peroxide and chloride ion.

European journal of biochemistry·2000
Same author

Peroxidase-catalyzed halide ion oxidation.

Redox report : communications in free radical research·2000
Same author

The reaction rates of NO with horseradish peroxidase compounds I and II.

Nitric oxide : biology and chemistry·2000
Same author

Kinetics of oxidation of serotonin by myeloperoxidase compounds I and II.

Biochemistry and cell biology = Biochimie et biologie cellulaire·1999

Peroxidases usually follow a standard reaction cycle, but exceptions exist. Prostaglandin H synthase exhibits unique peroxidase activity, linking its cyclooxygenase and peroxidase functions.

Area of Science:

  • Biochemistry
  • Enzymology

Background:

  • Peroxidases catalyze reactions via a multi-step cycle involving enzyme intermediates like compound I and compound II.
  • These steps typically involve hydrogen atom transfer from a substrate to the enzyme.

Purpose of the Study:

  • To elucidate the reaction mechanisms and intermediates of peroxidases.
  • To investigate the specific enzymatic activities and reaction cycles of Prostaglandin H synthase.

Main Methods:

  • The study focuses on the established reaction mechanisms of peroxidases.
  • Comparative analysis of Prostaglandin H synthase against typical peroxidase and P450 enzyme models.

Main Results:

  • Peroxidases generally proceed through native enzyme -> compound I -> compound II -> native enzyme stages.

Related Experiment Videos

  • Exceptions include electron transfer with substrate pi-cation radical formation, two-electron transfers, and involvement of compound III.
  • Prostaglandin H synthase demonstrates P450-like properties, with compound I abstracting hydrogen atoms from C-H bonds.

    • Conclusions:

    • The cyclooxygenase and peroxidase activities of Prostaglandin H synthase are closely linked.
    • These activities largely conform to a conventional peroxidase cycle, with noted exceptions in hydrogen atom abstraction.