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Published on: October 30, 2018
Vaccination with whole-cell vaccine and bacterial protein extract protects tilapia against Streptococcus difficile
A Eldar1, O Shapiro, Y Bejerano
1Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Abstract:
Formalin-killed Streptococcus difficile strains used as vaccines delivered intraperitoneally were able to protect tilapia against a challenge of 100 LD50. The protection obtained was not strain specific. A vaccine based on an S. difficile extract containing 50% protein conjugated to alum also protected tilapia challenged with a virulent S. difficile strain. Protection in tilapia was correlated with the development of specific agglutinins. Western blot analysis supported the hypothesis that only a few proteins act as protective antigens in both the whole-cell vaccine and the streptococcal extract. The high efficacy of these vaccines make them good candidates for the control of streptococcal fish meningoencephalitis.
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