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Updated: Aug 10, 2026

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Published on: February 9, 2009
Properties of the active plum pox potyvirus RNA polymerase complex in defined glycerol gradient fractions
M T Martin1, M T Cervera, J A Garcia
1Centro de Biologia Molecular (C.S.I.C.) Campus de la Universidad Autonoma de Madrid, Spain.
Abstract:
As a first step in the study of the replication of plum pox virus (PPV) RNA, an in vitro virus-specific RNA polymerase activity was characterized in a crude membrane extract (Martin and Garcia, 1991). In this study, we report the fractionation of the crude membrane extract by centrifugation in glycerol gradients. The sedimentation properties after different treatments of the crude extract and its insensitivity to micrococcal nuclease treatment suggest that the RNA polymerase activity was localized in a defined and enclosed membranous structure. Subcellular membrane characterization of the different glycerol gradient fractions indicated that PPV-specific RNA synthesis occurred in fractions enriched in endoplasmic reticulum and tonoplast vesicles.

