Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionomycin-stimulated phasic myometrial contractions

M Phillippe1, E M Chien, M Freij

  • 1Department of Obstetrics and Gynecology, University of Chicago, Illinois 60637, USA.

The American Journal of Physiology
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vascular Eagle's syndrome: difficult diagnosis in patient with recurrent transient ischaemic attack.

Annals of the Royal College of Surgeons of England·2026
Same author

A systematic review of ultrasonography-guided transcutaneous fine needle aspiration cytology in the diagnosis of laryngeal malignancy.

Annals of the Royal College of Surgeons of England·2024
Same author

Psychological interventions for acute psychiatric inpatients with schizophrenia-spectrum disorders: A systematic review and meta-analysis.

Clinical psychology review·2020
Same author

Generation of <i>Amelx-iCre</i> Mice Supports Ameloblast-Specific Role for <i>Stim1</i>.

Journal of dental research·2019
Same author

Adrenergic stimulation of diacylglycerol production in genital tract smooth muscle myocytes.

In vitro cellular & developmental biology. Animal·2016
Same author

Evaluating three hundred and fifty-two admissions and predictors of re-admissions for epistaxis - is it time to re-evaluate tranexamic acid in epistaxis?

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2015

Ionomycin, a calcium ionophore, triggers dose-related phasic contractions in myometrial tissue. This effect involves phospholipase C activation and calcium signaling pathways, similar to oxytocin.

Area of Science:

  • Pharmacology
  • Reproductive Biology
  • Cell Physiology

Background:

  • Ionomycin, a calcium ionophore, typically facilitates calcium entry.
  • Unexpectedly, ionomycin stimulates phasic contractions in myometrial tissue.
  • The underlying mechanisms for this myometrial response require further investigation.

Purpose of the Study:

  • To further define the effect of ionomycin on myometrial contractions.
  • To explore the potential mechanisms mediating ionomycin-induced myometrial contractions.
  • To investigate the role of phospholipase C in ionomycin's action.

Main Methods:

  • In vitro isometric contraction studies using rat uterine strips.
  • Administration of ionomycin with and without inhibitors of cytosolic calcium oscillations.

Related Experiment Videos

  • Qualitative inositol phosphate production assays to assess phospholipase C activity.

    • Main Results:

    • Ionomycin stimulated dose-related phasic myometrial contractions.
    • These contractions were potentially dependent on phospholipase C stimulation, calcium-induced calcium release, and L-type calcium channel influx.
    • Ionomycin increased inositol phosphate production in a dose-dependent manner, comparable to oxytocin.

    Conclusions:

    • Ionomycin induces dose-related phasic myometrial contractions.
    • The phosphatidylinositol signaling pathway, including phospholipase C activation, is implicated in ionomycin's effect.
    • These findings provide insight into the mechanisms of myometrial contractility.