Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell death in the olfactory epithelium

L Magrassi1, P P Graziadei

  • 1Department of Biological Science, Florida State University, Tallahassee 32306-4075, USA.

Anatomy and Embryology
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraocular transplants of olfactory neuroepithelium in rat.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2014
Same author

Acute transient inflammatory leukoencephalopathy in HIV.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2011
Same author

Total endoscopic approach to the cauda in a patient with a tight filum.

Minimally invasive neurosurgery : MIN·2008
Same author

Comparative expression profiles of ShcB and ShcC phosphotyrosine adapter molecules in the adult brain.

Neuroscience·2005
Same author

Lack of enantioselectivity of herpes virus thymidine kinase allows safer imaging of gene delivery.

Gene therapy·2003
Same author

Cell transplantation for Parkinson's disease in the age of deep brain stimulation.

Journal of neurosurgical sciences·2003

Olfactory neurons in rats undergo physiological cell death, indicated by DNA fragmentation and reduced RNA synthesis. This suggests a programmed cell death pathway for these sensory neurons.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Histology

Background:

  • The olfactory epithelium in vertebrates has unique cytology, with continuous olfactory neuron replacement throughout life.
  • The cause of olfactory neuron death (environmental insult vs. programmed cell death) is not fully understood.

Purpose of the Study:

  • To investigate the distribution and characteristics of cell death in the olfactory epithelium of normal adult rats.
  • To determine if olfactory neuron death is part of a physiological program.

Main Methods:

  • Microscopic examination (light and electron) of olfactory epithelium.
  • Vital staining with acridine orange.
  • Non-radioactive in situ labeling of fragmented DNA.
  • In vivo 5-bromouridine incorporation to assess RNA synthesis.

Related Experiment Videos

Main Results:

  • Pycnotic bodies, indicative of apoptosis, were observed in olfactory neurons.
  • Olfactory neuron nuclei showed chromatin condensation and increased cytoplasmic electron density.
  • Olfactory neurons were positive for fragmented DNA, unlike supporting and basal cells.
  • RNA synthesis was lower in olfactory neurons compared to other epithelial cells.

Conclusions:

  • Olfactory neurons exhibit morphological and molecular markers of programmed cell death.
  • These findings suggest olfactory neurons are predisposed to physiological cell death early in their development.