Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 13, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Development of the mouse B-cell repertoire

F Martin1, X Chen, F Shu

  • 1Department of Microbiology, University of Alabama at Birmingham 35294, USA.

Annals of the New York Academy of Sciences
|September 29, 1995
PubMed
Summary

Early mouse B cells show self-reactivity due to limited germline components. Selective pressures shape this B-cell repertoire, favoring some self-reactive cells while restricting others from maturing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Constitutive activation of mitogen-activated protein kinase-activated protein kinase 2 by mutation of phosphorylation sites and an A-helix motif.

The Journal of biological chemistry·1995
Same author

Use of tension band wires in horses with fractures of the ulna: 22 cases (1980-1992).

Journal of the American Veterinary Medical Association·1995
Same author

Effect of Beconase nasal spray on olfactory function in post-nasal polypectomy patients: a prospective controlled trial.

The Journal of laryngology and otology·1995
Same author

Participation of the propeptide on procathepsin D activation of human peripheral lymphocytes and neutrophils.

Archives of biochemistry and biophysics·1995
Same author

Expressed sequence tags of randomly selected cDNA clones from Eucalyptus globulus-Pisolithus tinctorius ectomycorrhiza.

Molecular plant-microbe interactions : MPMI·1995
Same author

[Gastric emphysema associated with gastric volvulus].

Gastroenterologia y hepatologia·1995

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • The early B-cell repertoire is crucial for immune system development.
  • B-cell receptor (BCR) diversity is generated through V(D)J recombination.
  • Understanding repertoire selection is key to immune tolerance.

Purpose of the Study:

  • To investigate the structural basis of early B-cell receptor (BCR) properties.
  • To elucidate the role of selective pressures on the developing B-cell repertoire.
  • To characterize the self-selective mechanisms acting on B cells in vivo.

Main Methods:

  • Analysis of BCR immunoglobulins in early ontogeny.
  • Utilizing VH81X transgenic mouse models.
  • In vivo studies to observe B-cell population dynamics.

More Related Videos

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Related Experiment Videos

Last Updated: Jul 13, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Main Results:

  • Early B cells exhibit high connectivity and self-reactivity, with low affinity BCRs.
  • Restricted germline components contribute to the structural basis of these BCRs.
  • In vivo models reveal dual selective pressures (positive and negative) on self-reactive B cells.
  • Specific self-reactive B cells are favored in the CD23-IgM+ population but excluded from the CD23+IgM+ compartment.

Conclusions:

  • The developing mouse B-cell repertoire is shaped by structural constraints and complex selective pressures.
  • Self-reactivity is a feature of early B cells, modulated by distinct positive and negative selection mechanisms.
  • These findings provide insights into the generation of a functional and self-tolerant B-cell repertoire.