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Isolation of lymphocyte populations

T G Pretlow

    National Cancer Institute Monograph
    |December 1, 1978
    PubMed
    Summary

    Lymphocytes are less dense and sediment slower than most mammalian cells. This study describes methods for separating lymphocytes from various tissues using velocity sedimentation.

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    Area of Science:

    • Cell Biology
    • Immunology
    • Biophysics

    Background:

    • Lymphocytes, crucial immune cells, exhibit heterogeneous physical properties.
    • Understanding lymphocyte physical characteristics is key for cell separation techniques.

    Purpose of the Study:

    • To describe methods for separating lymphocytes based on their physical properties.
    • To demonstrate the application of velocity sedimentation for lymphocyte isolation.

    Main Methods:

    • Utilizing velocity sedimentation in an isokinetic gradient.
    • Analyzing physical properties like cell size and density.
    • Separating lymphocytes from human blood and tumor tissues.

    Main Results:

    • Average lymphocyte density is lower than most mammalian cells.
    • Lymphocytes sediment slower than most mammalian cells due to their physical properties.
    • Successful separation of lymphocytes from diverse biological samples was achieved.

    Conclusions:

    • Lymphocyte physical properties, particularly density, influence their sedimentation rate.
    • Velocity sedimentation is an effective technique for isolating lymphocytes.
    • The described methods are applicable to various lymphocyte sources, including pathological samples.

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