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Peroxynitrite causes apoptosis in rat thymocytes

M G Salgo1, G L Squadrito, W A Pryor

  • 1Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800, USA.

Biochemical and Biophysical Research Communications
|October 24, 1995
PubMed
Summary

Peroxynitrite induces programmed cell death (apoptosis) in rat thymocytes by damaging DNA. This strong oxidant initiates genomic degradation, revealing a novel role for peroxynitrite in cellular apoptosis.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Toxicology

Background:

  • Peroxynitrite is a potent biological oxidant formed from superoxide and nitric oxide.
  • Its role in cellular damage is increasingly recognized.
  • Apoptosis is a critical process of programmed cell death.

Purpose of the Study:

  • To investigate the effect of peroxynitrite on rat thymocytes.
  • To determine if peroxynitrite can induce apoptosis.
  • To elucidate the mechanism of peroxynitrite-induced DNA damage.

Main Methods:

  • Freshly harvested rat thymocytes were suspended in phosphate buffer.
  • Treatment with peroxynitrite.
  • Analysis of DNA fragmentation using gel electrophoresis to detect the characteristic ladder pattern.

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Main Results:

  • Peroxynitrite induced apoptosis in rat thymocytes.
  • DNA fragmentation, a hallmark of apoptosis, was observed.
  • Evidence suggests peroxynitrite can directly nick DNA and oxidize cellular thiols.

Conclusions:

  • Peroxynitrite is capable of inducing apoptosis in thymocytes.
  • Peroxynitrite's ability to damage DNA contributes to its apoptotic effects.
  • This study identifies a novel function for peroxynitrite in initiating programmed cell death.