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Antisense oligonucleotide inhibits the expression of procollagen type III gene in mouse fibroblasts
Y Kitamura1, T Tanigawa, E Nanba
1Second Department of Pathology, Tottori University Faculty of Medicine, Yonago, Japan.
Biochemical and Biophysical Research Communications
|October 24, 1995
Abstract:
The effects of transfection of phosphorothioate antisense oligonucleotides (ASOs) into BALB/c 3T3 mouse fibroblasts on procollagen type III production were examined. Immunohistochemistry and Western blots showed that the expression of procollagen type III decreased. Semi-quantitative reverse transcription polymerase chain reaction revealed that the expression of procollagen type III mRNA was inhibited. ASOs were effective for inhibiting procollagen type III production at the gene level, although the effect was transient.