Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A designed beta-hairpin peptide

S K Awasthi1, S Raghothama, P Balaram

  • 1Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.

Biochemical and Biophysical Research Communications
|November 2, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Formylation of His Residues at Position 2 in Peptide b Ions due to Noncanonical C<sup>α</sup>-CO Bond Cleavage: Assignment of the m/z 166 Product Ion in Proteome Data.

Rapid communications in mass spectrometry : RCM·2025
Same author

Resurrection of the Helical Hairpin Hypothesis for Understanding Coronavirus Fusion.

The Journal of membrane biology·2025
Same author

Vitiligo detection capabilities of 1D photonic crystal biosensing design.

Scientific reports·2025
Same author

Deciphering the Conformations of Glutathione Oxidized Peptide: A Comparative NMR Study in Solution and Solid-State Environments.

Magnetic resonance in chemistry : MRC·2024
Same author

Ultra-high sensitive cancerous cells detection and sensing capabilities of photonic biosensor.

Scientific reports·2023
Same author

Ultra-sensitive pressure sensing capabilities of defective one-dimensional photonic crystal.

Scientific reports·2023
Same journal

A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

Biochemical and biophysical research communications·2026
Same journal

NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

Biochemical and biophysical research communications·2026
Same journal

Deciphering potent MPL activation by the fucose-binding lectin thrombocorticin.

Biochemical and biophysical research communications·2026
Same journal

Mitochondrial genome alterations in cancer: From mutations and epigenetics to targeted therapiesack.

Biochemical and biophysical research communications·2026
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
See all related articles

This study designed a synthetic octapeptide as a beta-hairpin model. Spectroscopic and NMR analyses confirmed its stable beta-sheet structure, showing potential for larger beta-sheet models.

Area of Science:

  • Peptide Chemistry
  • Structural Biology
  • Biophysical Chemistry

Background:

  • Beta-hairpin structures are crucial motifs in protein folding.
  • Understanding peptide conformation is key to designing novel biomaterials.
  • Synthetic peptides serve as valuable models for complex biological structures.

Purpose of the Study:

  • To design and synthesize a model octapeptide capable of adopting a beta-hairpin conformation.
  • To characterize the secondary structure and conformational stability of the synthetic peptide.
  • To explore the potential of this model for constructing larger beta-sheet assemblies.

Main Methods:

  • Peptide synthesis of Boc-Leu-Val-Val-D-Pro-Gly-Leu-Val-Val-OMe.
  • Circular dichroism (CD) spectroscopy in various organic solvents.

Related Experiment Videos

  • Nuclear Magnetic Resonance (NMR) spectroscopy (CDCl3, C6D6) including Nuclear Overhauser Effect (NOE) analysis.

    • Main Results:

    • CD spectra showed a negative band at 214-217 nm, indicative of beta-sheet structures.
    • NMR studies confirmed solvent-shielded NH groups (Leu(1), Val(3), Leu(6), Val(8)).
    • Interresidue NOEs provided strong evidence for a beta-hairpin conformation, including a Type II' beta-turn (D-Pro-Gly) and extended arms.

    Conclusions:

    • The synthetic octapeptide successfully models a beta-hairpin conformation.
    • The peptide exhibits high solubility in organic solvents.
    • This model holds promise for developing synthetic models of multi-stranded beta-sheets.