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Is polyamine decrease a common feature of apoptosis? Evidence from gamma rays- and heat shock-induced cell death

E Grassilli1, M A Desiderio, E Bellesia

  • 1Department of Biomedical Sciences, University of Modena, Italy.

Biochemical and Biophysical Research Communications
|November 13, 1995
PubMed
Summary
This summary is machine-generated.

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Apoptosis involves increased ornithine decarboxylase (ODC) activity and decreased polyamine levels in rat thymocytes. This polyamine metabolism imbalance appears to be a common feature of programmed cell death.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Apoptosis, or programmed cell death, is a crucial biological process.
  • Polyamines are essential for cell growth and proliferation.
  • The role of polyamine metabolism during apoptosis is not fully understood.

Purpose of the Study:

  • To investigate the changes in ornithine decarboxylase (ODC) activity and polyamine levels during apoptosis in rat thymocytes.
  • To determine if polyamine metabolism imbalance is a general feature of apoptosis.

Main Methods:

  • Rat thymocytes were induced to undergo apoptosis using heat shock and gamma irradiation.
  • Ornithine decarboxylase (ODC) mRNA accumulation and activity were measured.
  • Intracellular polyamine levels (putrescine, spermidine, spermine) were quantified.

Related Experiment Videos

  • DNA laddering was assessed as an indicator of apoptosis.

    • Main Results:

    • Heat shock and gamma irradiation induced significant ODC mRNA accumulation and activity in rat thymocytes.
    • Despite increased ODC activity, intracellular polyamine levels (putrescine, spermidine, spermine) decreased significantly before DNA laddering.
    • This suggests a rapid catabolism or altered utilization of polyamines during early apoptosis.

    Conclusions:

    • Early apoptosis in rat thymocytes is characterized by a marked activation of ODC and a simultaneous decrease in polyamine levels.
    • This imbalance in polyamine metabolism, with high ODC activity and low polyamine content, may be a conserved mechanism across different apoptotic stimuli.
    • These findings contribute to understanding the complex molecular events underlying programmed cell death.