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Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae is a glycoprotein
1Department of Food Science and Technology, Ohio State University, Columbus 43210, USA.
Biochemical and Biophysical Research Communications
|November 22, 1995
Abstract:
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using the highly specific periodate-digoxigenin antidigoxigenin method which is diagnostic for glycoproteins.