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Related Experiment Videos

Fas receptor expression on B-lineage cells

L Mandik1, K A Nguyen, J Erikson

  • 1Wistar Institute, Philadelphia 19104, USA.

European Journal of Immunology
|November 1, 1995
PubMed
Summary

Mice with the lpr mutation have defects in B and T cells. Fas receptor (FasR) expression is present on most B cells, but absent in lpr/lpr mice, indicating an intrinsic B cell defect.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The lpr mutation in mice leads to B and T cell defects and autoantibody development.
  • This defect is linked to the apoptosis-associated Fas receptor (FasR) gene.
  • Understanding FasR's role in B cells is crucial for autoimmune disease research.

Purpose of the Study:

  • To investigate the expression of FasR on B-lineage cells throughout their development.
  • To determine if the lpr mutation affects FasR expression in B cells.

Main Methods:

  • Surveying FasR expression on B-lineage cells from bone marrow progenitors to peripheral B cells.
  • Comparing FasR expression in wild-type and lpr/lpr mice.

Main Results:

  • FasR is expressed on B cells at all developmental stages, with highest levels on germinal center B cells.
  • FasR is notably absent on B cells derived from lpr/lpr mice.
  • B-1 (CD5) B cells do not constitutively express FasR; expression is induced upon activation.

Conclusions:

  • The lpr mutation causes an intrinsic defect in B cells, affecting their FasR expression.
  • FasR plays a significant role in B cell development and function.
  • The differential expression of FasR on B-1 cells suggests unique regulatory mechanisms for this subset.

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