A phage display vector with improved stability, applicability and ease of manipulation
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B C Courtney1, K C Williams, J J Schlager
1Applied Pharmacology Branch, United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425, USA.
Abstract:
We modified a combinatorial library display vector, pCOMB3, to provide a stable, easily manipulated, high-copy vector for the display of a random hexapeptide library. The propensity of the original phagemid to accumulate 800-1000-bp deletions in the region of the cloning site has been eliminated. Furthermore, the small 63-bp 'stuffer' at the cloning site was replaced with a 2114-bp DNA fragment from adenovirus 2. This produced a 5808-bp vector, that we have named pICD1LS, with the appropriate characteristics for single-peptide phage display. Libraries of greater than 10(6) molecules were produced with this vector.