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Related Experiment Videos

Hormonal regulation of caveolae internalization

E J Smart1, Y S Ying, R G Anderson

  • 1Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.

The Journal of Cell Biology
|November 1, 1995
PubMed
Summary

The caveolae internalization cycle relies on protein kinase C-alpha (PKC-alpha) phosphorylating a 90-kD protein. This process is regulated by diacylglycerol and histamine, suggesting hormonal control over caveolae dynamics.

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Area of Science:

  • Cell Biology
  • Membrane Trafficking
  • Signal Transduction

Background:

  • Caveolae are dynamic membrane structures involved in cellular processes.
  • Their cyclic transition from flat to vesicular forms is crucial for cell function.

Purpose of the Study:

  • To investigate the molecular mechanisms regulating caveolae internalization.
  • To identify key proteins and signaling pathways involved in caveolae dynamics.

Main Methods:

  • Studied caveolae internalization in cells treated with phorbol-12,13-dibutyrate, diacylglycerol, histamine, pyrilamine, and cimetidine.
  • Assessed the role of protein kinase C-alpha (PKC-alpha) and a 90-kD protein phosphorylation.
  • Investigated the presence and function of a protein phosphatase in caveolae.

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Main Results:

  • PKC-alpha in caveolae phosphorylates a 90-kD protein, essential for the internalization cycle.
  • Displacement of PKC-alpha by diacylglycerol or histamine inhibits phosphorylation and internalization.
  • A protein phosphatase dephosphorylates the 90-kD protein.
  • Histamine-induced inhibition involves histamine H1 receptors.

Conclusions:

  • The caveolae internalization cycle is dependent on PKC-alpha-mediated phosphorylation of a 90-kD protein.
  • This cycle is regulated by intracellular diacylglycerol levels and extracellular histamine signaling.
  • Caveolae internalization is under hormonal control via histamine H1 receptors.