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Updated: Jul 9, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Quantitation of hepatitis C viral RNA in liver and serum samples using competitive polymerase chain reaction
12nd Department of Internal Medicine, Faculty of Medicine, Kobe University, Japan.
Aims:
To investigate whether the amount of hepatitis C viral RNA (HCV-RNA) in liver and serum can predict the effectiveness of interferon treatment in patients with chronic hepatitis C.
Methods:
The amount of HCV-RNA in frozen liver tissues and sera of 22 patients with chronic hepatitis C was determined before and after interferon alfa treatment by the competitive reverse transcriptional polymerase chain reaction method.
Results:
Patients with small amounts of HCV-RNA in serum before treatment showed a significantly more effective response to interferon treatment. After treatment, HCV-RNA disappeared from both the liver and serum of all patients who sustained complete response (n = 11); in contrast, HCV-RNA decreased but persisted in the liver of all those who relapsed after cessation of treatment (n = 11).
Conclusions:
The elimination of hepatitis C virus from the liver as well as from the serum seems to be essential for sustained remission. The quantitation of HCV-RNA in liver biopsy specimens obtained after treatment would be a highly accurate predictor of whether relapse is likely to occur.
