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Identification of parietal cells in gastric body mucosa with HMFG-2 monoclonal antibody
M M Walker1, A Smolka, J M Waller
1Department of Histopathology, St Mary's Hospital Medical School, London.
Aims:
To identify parietal cells in the upper gastrointestinal tract by an immunoperoxidase method, using commercially available monoclonal antibodies.
Methods:
Routine surgical biopsy specimens of gastric body mucosa were examined using the avidin-biotin peroxidase method with the monoclonal antibodies HMFG-1 and HMFG-2 to identify parietal cells. Double immunoperoxidase labelling with HK12.18, a well characterised monoclonal antibody directed against an epitope on the alpha (catalytic) subunit of H+ translocating, K+ stimulated adenosine triphosphatase (H,K-ATPase), was used to confirm that HMFG-1 and -2 stained parietal cells.
Results:
HMFG-1 and HMFG-2 showed consistent parietal cell staining patterns in the gastric body mucosa. HMFG-2 gave a more intense staining pattern of the secretory canaliculi. This was confirmed by double immunolabelling with HK12.18.
Conclusions:
HMFG monoclonal antibodies are recommended as highly specific markers of human gastric parietal cells.
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