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Updated: Jul 21, 2026
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
Evidence for E-selectin complement regulatory domain mRNA splice variants in the rat
K L Billups1, J L Sherley, M A Palladino
1Department of Urologic Surgery, University of Minnesota, Minneapolis 55455, USA.
The Journal of Laboratory and Clinical Medicine
|December 1, 1995
Summary
Researchers discovered two forms of E-selectin mRNA in rats, differing in a complement regulatory domain (CR5). The CR5-negative variant was more abundant, suggesting distinct roles in inflammation.
Area of Science:
- Molecular Biology
- Immunology
- Biochemistry
Background:
- E-selectin mediates endothelial cell-leukocyte interactions in acute inflammation.
- Lipopolysaccharide (LPS) is a potent inducer of acute inflammation.
Purpose of the Study:
- Investigate the molecular regulation of E-selectin function.
- Examine E-selectin mRNA expression in rat tissues following LPS administration.
Main Methods:
- Isolated two unique rat E-selectin cDNA fragments.
- Analyzed nucleotide sequence homology with mouse and human E-selectin.
- Detected and quantified mRNA variants in rat heart tissue via RT-PCR.
Main Results:
- Identified two rat E-selectin cDNA fragments differing in complement regulatory domain-5 (CR5) sequences.
- Demonstrated the existence of E-selectin CR-variant mRNAs within the same species.
- Observed that both CR5(+) and CR5(-) mRNA variants were induced by LPS, with CR5(-) being more abundant.
Conclusions:
- This study provides the first direct evidence of E-selectin CR-variant mRNAs in rats.
- The differential abundance of CR5(-) mRNA suggests distinct functional roles in E-selectin-mediated inflammatory processes.
- CR5 variant proteins may perform functionally distinct tasks in inflammation.
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