Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetic and functional mapping of viral epitopes using biosensor technology

H Saunal1, M H Van Regenmortel

  • 1Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Strasbourg, France.

Virology
|November 10, 1995
PubMed
Summary

Certain monoclonal antibodies (mAbs) targeting tobacco mosaic virus (TMV) can block viral disassembly by ribosomes. Inhibitory mAbs bind to a specific site on the viral protein, preventing ribosome interaction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Opportunities for training in microbiology adapted to the needs of Third World countries.

World journal of microbiology & biotechnology·2014
Same author

Emulsification of vegetable oils: application to olive oil.

International journal of cosmetic science·2009
Same author

Influence of homogenization on emulsion stability.

International journal of cosmetic science·2009
Same author

Stabilisation of emulsions: influence of hydrophilic colloids.

International journal of cosmetic science·2009
Same author

Major isomers of the active ingredient of the sunscreen 5(3,3-dimethyl-2-norbornylidene)-3 pentene-2-one.

International journal of cosmetic science·2009
Same author

The potential of retro-inverso peptides as synthetic vaccines.

Expert opinion on investigational drugs·2005

Area of Science:

  • Virology
  • Immunology
  • Biochemistry

Background:

  • Tobacco mosaic virus (TMV) disassembly by ribosomes is crucial for its infection cycle.
  • Monoclonal antibodies (mAbs) can target viral components, potentially interfering with viral processes.
  • Understanding antibody-epitope interactions is key to developing antiviral strategies.

Purpose of the Study:

  • To investigate the mechanism by which certain mAbs inhibit TMV disassembly by ribosomes.
  • To map the binding sites of inhibitory and non-inhibitory mAbs on the TMV coat protein.
  • To evaluate the utility of biosensor technology in characterizing antibody-epitope interactions on viral proteins.

Main Methods:

  • Kinetic two-site binding assays using biosensor technology (BIAcore) were employed.

Related Experiment Videos

  • Monoclonal antibodies (mAbs) were tested for their ability to inhibit TMV disassembly.
  • Epitope mapping was performed on the viral monomeric protein (TMVP).

    • Main Results:

    • Some mAbs blocked TMV disassembly, while others did not, with no correlation to binding kinetics or affinity.
    • Inhibitory mAbs bound to a specific region of the TMVP surface near the central axis.
    • This binding site is implicated in the interaction between the viral protein and RNA.

    Conclusions:

    • Inhibitory mAbs likely act by sterically hindering the interaction between TMV and ribosomes.
    • Biosensor technology is effective for locating conformational epitopes on viral proteins.
    • The findings provide insights into TMV-ribosome interactions and potential antiviral targets.