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Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA
M Maccarrone1, M P Hilbers, G A Veldink
1Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy.
Abstract:
A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
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