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In vitro antibacterial activity of florfenicol against Actinobacillus pleuropneumoniae
1Animal Health Research Laboratories, Agro Division, Takeda Chemical Industries, Ltd., Kyoto, Japan.
Abstract:
A total of 90 strains of Actinobacillus pleuropneumoniae isolated from porcine pneumonic lungs in Japan during 1989 to 1993 were investigated for their in vitro susceptibility to florfenicol (a derivative of thiamphenicol) and other drugs. The minimal inhibitory concentrations of florfenicol against A. pleuropneumoniae ranged from 0.2 to 1.56 micrograms/ml with a peak at 0.39 microgram/ml. Florfenicol showed a high antibacterial activity against A. pleuropneumoniae regardless of the sensitivity of the strains to thiamphenicol.
Insights
Florfenicol demonstrates potent in vitro antibacterial activity against Actinobacillus pleuropneumoniae, a common swine pathogen. This study confirms florfenicol
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
- Swine Diseases
Background:
- Actinobacillus pleuropneumoniae is a significant bacterial pathogen causing pleuropneumonia in pigs.
- Antimicrobial susceptibility testing is crucial for guiding effective treatment strategies.
- Florfenicol, a thiamphenicol derivative, is used in veterinary medicine.
Purpose of the Study:
- To evaluate the in vitro susceptibility of Actinobacillus pleuropneumoniae strains to florfenicol.
- To compare florfenicol's efficacy with other antimicrobial agents.
- To assess the impact of thiamphenicol sensitivity on florfenicol activity.
Main Methods:
- Isolation of 90 Actinobacillus pleuropneumoniae strains from porcine pneumonic lungs in Japan (1989-1993).
- Determination of minimal inhibitory concentrations (MICs) for florfenicol using standard microbiological techniques.
- Assessment of florfenicol activity in relation to thiamphenicol sensitivity.
Main Results:
- Florfenicol exhibited high in vitro activity against all tested Actinobacillus pleuropneumoniae strains.
- MIC values for florfenicol ranged from 0.2 to 1.56 µg/ml, with a peak at 0.39 µg/ml.
- Florfenicol efficacy remained consistent regardless of the strains' sensitivity to thiamphenicol.
Conclusions:
- Florfenicol is a highly effective antimicrobial agent against Actinobacillus pleuropneumoniae.
- Florfenicol provides a reliable treatment option for swine pleuropneumonia.
- Its effectiveness is not compromised by existing thiamphenicol resistance patterns.