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High-performance liquid chromatographic determination of prolylcarboxypeptidase activity in monkey kidney
Y Suzawa1, B Y Hiraoka, M Harada
1Department of Orthodontics, Matsumoto Dental College, Shiojiri-Nagano, Japan.
Journal of Chromatography. B, Biomedical Applications
|August 4, 1995
Abstract:
A simple HPLC procedure for the determination of prolylcarboxypeptidase activity in monkey kidney was established with Cbz-Pro-Ala used as substrate. Decrease of the substrate and increase of the product were stoichiometrically related to each other. Heat treatment at 60 degrees C freed the enzyme preparation of contaminating activities. Data on substrate specificity and influence of inhibitors suggested this method was sensitive for the determination of prolylcarboxypeptidase without the use of a radioactive substrate.