Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Designing human consensus antibodies with minimal positional templates

J R Couto1, R B Christian, J A Peterson

  • 1Cancer Research Fund of Contra Costa, Walnut Creek, California 94596, USA.

Cancer Research
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Performance of Sotatercept in PAH Patients With Cardiopulmonary Comorbidities: A Retrospective Single Center Experience.

Pulmonary circulation·2025
Same author

Conversion of Site-Specific Meteorological Data for use in CAP-88 PC.

Health physics·2024
Same author

Anxiety-associated and separation distress-associated behaviours in Angelman syndrome.

Journal of intellectual disability research : JIDR·2019
Same author

Heparin-protamine balance after neonatal cardiopulmonary bypass surgery.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Women's Response to Army Training.

The Physician and sportsmedicine·2016
Same author

Visualization of Flow Behavior in Earth Mantle Convection.

IEEE transactions on visualization and computer graphics·2015
Same journal

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same journal

A Clinically Integrated Pediatric Patient-Derived Xenograft Program Enables Evaluation of Cohort and Patient-Specific Biology and Therapeutic Strategies.

Cancer research·2026
Same journal

Editor's Note: Heterodimerization of Insulin-like Growth Factor Receptor/Epidermal Growth Factor Receptor and Induction of Survivin Expression Counteract the Antitumor Action of Erlotinib.

Cancer research·2026
Same journal

Editor's Note: Deguelin Analogue SH-1242 Inhibits Hsp90 Activity and Exerts Potent Anticancer Efficacy with Limited Neurotoxicity.

Cancer research·2026
Same journal

Retraction: Two Functional Epitopes of Pigment Epithelial-Derived Factor Block Angiogenesis and Induce Differentiation in Prostate Cancer.

Cancer research·2026
Same journal

Editor's Note: Chronic Stress Facilitates Lung Tumorigenesis by Promoting Exocytosis of IGF2 in Lung Epithelial Cells.

Cancer research·2026
See all related articles

Developing a minimal positional template aids in selecting optimal human frameworks for antibody humanization, ensuring antigen binding integrity and minimizing immunogenicity. This method refines antibody engineering for therapeutic applications.

Area of Science:

  • Immunology
  • Biotechnology
  • Protein Engineering

Background:

  • Humanized antibodies retain antigen-binding regions from murine antibodies but can elicit immune responses due to differing framework residues.
  • Choosing appropriate human consensus frameworks is crucial for successful antibody humanization.
  • Existing methods require refinement to select optimal human frameworks for variable light and heavy chains.

Purpose of the Study:

  • To develop a minimal positional template for selecting human consensus frameworks during antibody humanization.
  • To ensure the integrity of antigen-binding domains while minimizing potential immunogenicity.
  • To refine the process of humanizing murine antibodies for therapeutic use.

Main Methods:

  • Developing a minimal positional template to identify critical framework positions for antigen binding.

Related Experiment Videos

  • Screening human consensus sequences for similarity to murine sequences at template-indicated positions.
  • Applying computer modeling and laboratory testing in iterative cycles to refine the template.

    • Main Results:

    • A conservative positional template successfully humanized antibodies (BrE-3, KC4-G3) without affinity loss.
    • Template B, derived from initial modeling, was successfully used for humanizing the Mc3 antibody.
    • Template C was designed, further refining the minimal template approach.

    Conclusions:

    • The minimal positional template approach is a novel and effective strategy for antibody humanization.
    • This method ensures retention of binding affinity and reduces potential immunogenicity.
    • Further validation of template C and the humanization approach is ongoing.