Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Are growth factors chemotactic agents?

D Zicha1, G A Dunn

  • 1MRC Muscle and Cell Motility Unit, Randall Institute, King's College London, United Kingdom.

Experimental Cell Research
|December 1, 1995
PubMed
Summary

Malignant T15 cells exhibit chemotaxis, releasing a chemoattractant that influences both T15 and K2 cells. This finding questions the direct chemotactic role of growth factors in this cancer cell system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coherence controlled holographic microscopy - a tool for detection of new biomarkers of head and neck squamous cell carcinoma.

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti·2022
Same author

Rediscovering contact inhibition in the embryo.

Journal of microscopy·2013
Same author

An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis.

Oncogene·2011
Same author

Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy.

Journal of microscopy·2008
Same author

Quantitative fluorescence resonance energy transfer (FRET) measurement with acceptor photobleaching and spectral unmixing.

Journal of microscopy·2004
Same author

Two dynamic morphotypes of sarcoma cells, asymmetric stellate and triangle with leading lamella, are related to malignancy.

Folia biologica·2003

Area of Science:

  • Cell Biology
  • Cancer Research
  • Chemotaxis

Background:

  • Chemotaxis is crucial for cell migration in various biological processes.
  • Understanding cancer cell migration is vital for developing effective treatments.
  • Previous studies have suggested growth factors as chemoattractants for malignant cells.

Purpose of the Study:

  • To directly observe and characterize chemotaxis in malignant sarcoma cells.
  • To investigate the role of platelet-derived and insulin-like growth factors in cancer cell migration.
  • To determine the chemoattractant properties of malignant cells themselves.

Main Methods:

  • Utilized a direct-viewing chemotaxis chamber with concentric wells.
  • Employed two rat sarcoma cell lines: highly malignant T15 and less malignant K2.
  • Analyzed pooled and individual cell trajectories in response to growth factors and other cells.

Main Results:

  • Highly malignant T15 cells showed biased migration towards growth factors.
  • Individual T15 cell trajectories displayed significant directional bias, deviating from the gradient.
  • Less malignant K2 cells responded to T15 cells, not directly to growth factors.
  • T15 cells released a chemoattractant that affected both T15 and K2 cells, overriding growth factor effects.

Conclusions:

  • Stimulated T15 cells release an endogenous chemoattractant for both cell types.
  • The observed chemoattractant from T15 cells overrides the effect of tested growth factors.
  • Direct observation is essential to ascertain the true chemoattractant nature of substances in complex biological systems.

Related Experiment Videos