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Auxiliary method for clonal identification of Staphylococcus aureus by protein band pattern of released proteins on
1Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan.
Microbiology and Immunology
|January 1, 1995
Abstract:
A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surface culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration procedure prior to electrophoresis. The combined use of cellophane surface culture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization.