[Comparison of procedures for determining Michaelis-Menten parameters together with their standard deviations using...

The direct non-linear fit of Cleland and different methods applying linear regression analysis to linearized forms of the Michaelis-Menten equation have been compared. Comparison was made with respect to bias and standard deviation of the estimated values of Km, Vm, deltak and deltav. The Lineweaver-Burk method, weighted by approximately v3, turned out to produce estimates nearly as reliable as the direct fit of Cleland if one does not know whether the velocities are subject to constant absolute or relative errors. The comparison was based on ca. 10000 simulated series of velocity measurements. Runs with fewer simulated measurement series showed that for comparing different evaluation methods, at least 1000 measurement series should be simulated in order to get reliable results.