Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of Applied Biosystems 373 sequencer stretch modification

L A McDonald1, J M Kelley, R C Brandon

  • 1Institute for Genomic Research, Gaithersburg, MD, USA.

Biotechniques
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Canadian Surgery Forum.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.

Journal of applied microbiology·2017
Same author

High prevalence of fasciolosis and evaluation of drug efficacy against Fasciola hepatica in dairy cattle in the Maffra and Bairnsdale districts of Gippsland, Victoria, Australia.

Veterinary parasitology·2015
Same author

Placebo effect in burning mouth syndrome: a systematic review.

Oral diseases·2013
Same author

The malaria genome sequencing project.

Protist·2012
Same author

BRM and BRG1 subunits of the SWI/SNF chromatin remodelling complex are downregulated upon progression of benign skin lesions into invasive tumours.

The British journal of dermatology·2011
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

Optimizing DNA sequencing methods on Applied Biosystems 373 Automated DNA Sequencers improves data accuracy. New gel conditions and optimized reactions yield longer, more reliable DNA reads for double-stranded DNA sequencing.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Applied Biosystems 373 Automated DNA Sequencers are widely used for DNA sequencing.
  • Standard protocols can lead to inaccuracies in DNA reads, particularly within the first 100 bases and for longer sequences (>400 bases).
  • Uneven fragment spacing and suboptimal signal-to-noise ratios impact data quality.

Purpose of the Study:

  • To optimize the Stretch modification for Applied Biosystems 373 Automated DNA Sequencers.
  • To improve DNA sequencing accuracy, read length, and data reliability for double-stranded DNA.
  • To enhance fragment spacing and signal strength in sequencing reactions.

Main Methods:

  • Tested various gel concentrations and wattage settings for Stretch modification on 34-cm and 48-cm configurations.

Related Experiment Videos

  • Optimized Taq dye primer cycle sequencing reactions using Applied Biosystems CATALYST 800 Molecular Biology LabStation.
  • Utilized -21 M13 and M13RP1 primers for optimized sequencing reactions.

    • Main Results:

    • Increased acrylamide concentration and wattage improved base spacing and fragment resolution.
    • Optimized reactions produced more even distribution of dye-labeled fragments, enhancing signal strength and reducing background.
    • The optimized conditions resulted in longer DNA reads with increased reliability and accuracy.

    Conclusions:

    • The optimized Stretch modification and reaction conditions significantly enhance DNA sequencing performance on Applied Biosystems 373.
    • This improved methodology provides more accurate and reliable data for double-stranded DNA sequencing applications.
    • The findings offer a practical solution for overcoming common limitations in automated DNA sequencing.