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An aberrant lck mRNA in two human T-cell lines
L B Vogel1, R Arthur, D J Fujita
1CNRS, Station Biologique, Roscoff, France.
Biochimica Et Biophysica Acta
|November 7, 1995
Summary
Researchers discovered an unusual intron in the src-family tyrosine kinase p56lck (lymphocyte-specific protein tyrosine kinase) in human T-cells. This aberrant lck RNA was also found in leukemic T-cell lines.
Area of Science:
- Molecular Biology
- Immunology
- Oncology
Background:
- The src-family tyrosine kinase p56lck is crucial for T-cell receptor signaling.
- Aberrant gene expression is implicated in T-cell malignancies.
Purpose of the Study:
- To investigate the molecular basis of T-cell signaling in human T-cells.
- To identify novel genetic variations in T-cell leukemia.
Main Methods:
- Low stringency screening of a human T-cell cDNA library using a c-src probe.
- Sequence analysis of isolated cDNA clones.
- Northern blotting and PCR amplification of poly(A)+ RNA from human leukemic T-cell lines.
Main Results:
- Isolation of partial cDNAs encoding the src-family tyrosine kinase p56lck.
- Identification of a novel 93 bp intron insertion within the aberrant lck cDNA.
- Detection of this aberrant lck RNA in human leukemic T-cell lines.
Conclusions:
- A novel aberrant form of lck RNA containing an intron insertion exists in human T-cells.
- This aberrant lck RNA is present in human leukemic T-cell lines, suggesting a potential role in T-cell malignancies.