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The Cowdria ruminantium groE operon

N C Lally1, S Nicoll, E A Paxton

  • 1Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Roslin, UK.

Microbiology (Reading, England)
|September 1, 1995
PubMed
Summary

Researchers identified Cowdria ruminantium heat shock proteins GroEL and GroES. The GroEL protein shows potential for developing specific diagnostic tests for Cowdria ruminantium infection, avoiding cross-reactions with Ehrlichia species.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Veterinary Microbiology

Background:

  • Cowdria ruminantium is an economically significant tick-borne intracellular bacterium affecting ruminants.
  • Accurate diagnostics are crucial for controlling Cowdria ruminantium infections.

Purpose of the Study:

  • To clone and characterize Cowdria ruminantium heat shock protein genes.
  • To evaluate the potential of these proteins for serodiagnostic applications.

Main Methods:

  • Construction of a Cowdria ruminantium genomic DNA library in lambda ZAPII.
  • Screening for immunoreactive clones using infected goat serum.
  • DNA sequencing and database searching.
  • Western blotting with antisera against Cowdria ruminantium and Ehrlichia species.

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Main Results:

  • Isolation and characterization of clone lambda Cr9.4 containing Cowdria ruminantium groES and groEL homologues.
  • The recombinant GroEL protein reacted with sera from C. ruminantium-infected animals.
  • No cross-reactivity was observed with antisera to closely related Ehrlichia species.

Conclusions:

  • The Cowdria ruminantium GroEL protein is a potential candidate for developing specific serodiagnostic tests.
  • This finding could improve the diagnosis of Cowdria ruminantium infections and differentiate them from Ehrlichia infections.
  • Cloning the GroE operon facilitates further research into Cowdria ruminantium immunobiology.