Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular basis for serological cross-reactivity between enteroviruses

A Samuelson1, M Forsgren, B Johansson

  • 1Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge Hospital, Sweden.

Clinical and Diagnostic Laboratory Immunology
|May 1, 1994
PubMed
Summary

Human antibodies cross-reacting between enteroviruses target conserved VP1 protein residues 42-55. This finding explains antibody cross-reactivity and provides a molecular basis for enterovirus immunity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Limited spread of hepatitis C among HIV-negative men who have sex with men in Stockholm, Sweden.

International journal of STD & AIDS·2013
Same author

Impact of IgG replacement therapy and antibiotic treatment on the colonization of non-encapsulated Haemophilus influenzae in the nasopharynx in patients with hypogammaglobulinaemia.

Scandinavian journal of infectious diseases·2002
Same author

Children with recurrent episodes of acute otitis media: the effect of local administration of immunoglobulin G on acute otitis media, colonization and turnover of non-encapsulated Haemophilus influenzae in the nasopharynx.

Clinical otolaryngology and allied sciences·2000
Same author

Intraoral digital imaging documenting recurrent aphthous ulcer healing in 2-octyl cyanoacrylate versus sham-treated lesions.

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics·2000
Same author

Development and application of a new method for amplification and detection of human rhinovirus RNA.

Journal of virological methods·1998
Same author

Detection of JC virus in cerebrospinal fluid (CSF) samples from patients with progressive multifocal leukoencephalopathy but not in CSF samples from patients with herpes simplex encephalitis, enteroviral meningitis, or multiple sclerosis.

Journal of clinical microbiology·1998

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Enteroviruses are a significant cause of human disease.
  • Antibody cross-reactivity between different enteroviruses is a known phenomenon.
  • The specific recognition sites responsible for this cross-reactivity have not been fully characterized.

Purpose of the Study:

  • To determine and characterize the recognition sites for human antibodies that cross-react between different enteroviruses.
  • To identify the specific amino acid residues within the viral capsid protein VP1 that are critical for this cross-reactivity.

Main Methods:

  • Enzyme immunoassays were performed using serum samples from patients with enteroviral infections.
  • Overlapping synthetic peptides covering regions of poliovirus 1 VP1 and coxsackievirus B1 VP1 were used.

Related Experiment Videos

  • Peptide analogs with specific residue substitutions were employed to pinpoint essential amino acids.

    • Main Results:

    • A major antigenic region eliciting cross-reactive antibodies was located to residues 37-51 of VP1.
    • A peptide spanning residues 42-55 of VP1 significantly inhibited antibody binding.
    • Specific residues (Pro-42, Ala-43, Leu-44, Thr-45, Ala-46, Glu-48, Thr-49, Gly-50) within this region were identified as essential for antibody binding.

    Conclusions:

    • The essential residues for binding of cross-reactive antibodies are highly conserved within the enterovirus family, with notable exceptions like echovirus 22.
    • These findings provide a molecular basis for understanding enterovirus antibody cross-reactivity.
    • This knowledge can inform the development of broader-acting antiviral strategies.