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Characterizing allelic proteins for genome mapping in maize

P Touzet1, C Morin, C Damerval

  • 1Station de Génétique Végétale, Université Paris XI/INRA/CNRS URA 1492, INAP-G, Gif-sur-Yvette, France.

Electrophoresis
|July 1, 1995
PubMed
Summary

Genetic linkage maps are crucial for gene isolation in plants. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) helps identify expressed genes by analyzing protein position shifts, aiding maize genome mapping.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • cDNA probes are widely used for genome analysis and gene isolation in cultivated plants.
  • Identifying expressed genes can be challenging as cDNA probes often detect multiple unlinked loci.

Purpose of the Study:

  • To evaluate two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) as a method for identifying expressed genes in maize.
  • To confirm if protein position shifts observed in 2-D PAGE correspond to allelic variations in structural genes.

Main Methods:

  • Analysis of segregating maize populations to study protein position shifts on 2-D gels.
  • Comparison of allelic proteins using high-performance liquid chromatography (HPLC) and partial amino acid sequencing.

Main Results:

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  • Previously established that protein position shifts on 2-D gels are monogenic and codominant.
  • In a sample of 20 position shifts, allelic proteins were found to be highly similar, with only one exception.
  • This suggests that polymorphism in structural genes is the primary cause of observed protein shifts.

Conclusions:

  • 2-D PAGE is a valuable tool for maize genome mapping.
  • Protein position shifts can help identify specific genes being translated when cDNA probes detect multiple loci.