Serological and cultural studies on broiler breeder multiplier flocks infected with Mycoplasma synoviae

Nine broiler breeder flocks found infected with Mycoplasma synoviae (MS) at 17 to 42 weeks of age were used for serological tests and isolations of MS. Five live females and 20 to 30 serums were brought to the laboratory from each flock on three occasions. Serums from flocks naturally infected reacted to the serum plate agglutination and hemagglutination-inhibition tests throughout one laying season. MS was isolated from the trachea, turbinates, sinus, lung, and air sacs throughout one laying season. The medium with 0.1 g NAD isolated MS more efficiently than the medium with 0.01 g NAD. MS was isolated more frequently from the trachea, turbinates, and sinus than from the lung and air sacs.