Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photoaffinity labeling of a cell surface polyamine binding protein

D M Felschow1, J MacDiarmid, T Bardos

  • 1Grace Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.

The Journal of Biological Chemistry
|December 1, 1995
PubMed
Summary

Researchers identified potential polyamine binding proteins on leukemia cell surfaces. These proteins may be involved in polyamine transport, a crucial cellular process for maintaining intracellular polyamine levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergism of small molecules targeting VDAC with sorafenib, regorafenib or lenvatinib on hepatocarcinoma cell proliferation and survival.

European journal of pharmacology·2023
Same author

Increased Helicobacter pylori-associated gastric cancer risk in the Andean region of Colombia is mediated by spermine oxidase.

Oncogene·2014
Same author

Meal and snacking patterns of school-aged children in Scotland.

European journal of clinical nutrition·2009
Same author

Polyamine biosynthesis impacts cellular folate requirements necessary to maintain S-adenosylmethionine and nucleotide pools.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2009
Same author

Assessing dietary intake: Who, what and why of under-reporting.

Nutrition research reviews·2008
Same author

Spermine and spermidine mediate protection against oxidative damage caused by hydrogen peroxide.

Amino acids·2007

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Intracellular polyamine concentrations are regulated by active transport systems.
  • Mammalian polyamine transport activity is known, but the specific proteins involved remain unidentified.

Purpose of the Study:

  • To identify proteins involved in polyamine transport using photoaffinity labeling.
  • To characterize polyamine binding proteins on the surface of leukemia cells.

Main Methods:

  • Photoaffinity labeling of murine L1210 and human U937 leukemia cells using synthesized spermidine and norspermine conjugates.
  • Separation and analysis of labeled plasma membrane proteins by SDS-PAGE.
  • Competition assays with polyamines and analogs to confirm labeling specificity.

Related Experiment Videos

  • Neuraminidase treatment and analysis of transport-deficient cells.

    • Main Results:

    • Two major proteins (p118 and p50) were heavily labeled by the spermidine photoprobe in L1210 cells.
    • Similar proteins were observed in U937 cells.
    • Labeling specificity was confirmed by competition assays and use of different photoprobes.
    • p118 showed increased mobility after neuraminidase treatment, indicating glycosylation and plasma membrane localization.
    • Transport-deficient cells exhibited altered protein profiles, suggesting a link to polyamine transport.

    Conclusions:

    • A polyamine binding protein, potentially p118, is present on the surface of leukemia cells.
    • This protein is likely involved in or related to the polyamine transport apparatus.
    • Further investigation is needed to confirm the direct role of these proteins in polyamine transport.