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Related Experiment Videos

Differential expression of alternate mB7-2 transcripts

F Borriello1, J Oliveros, G J Freeman

  • 1Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|December 15, 1995
PubMed
Summary

Murine B7-2 (mB7-2) costimulatory molecule transcripts are differentially regulated. Alternative splicing of exons 1-5 produces distinct mB7-2 transcripts, impacting immune responses.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Murine B7-2 (mB7-2) is a costimulatory molecule on antigen-presenting cells (APCs) crucial for T cell activation and anergy.
  • mB7-2 mRNA expression is restricted, inducible in B cells, T cells, NK cells, and dendritic cells, and constitutive in monocytes.

Purpose of the Study:

  • To characterize mB7-2 transcripts and analyze the genomic organization of the mB7-2 gene.
  • To understand the differential regulation of mB7-2 expression in various APCs.

Main Methods:

  • 5' rapid amplification of cDNA ends (RACE) to identify transcript variants.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze transcript expression.
  • Genomic analysis of the mB7-2 gene structure.

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Main Results:

  • The mB7-2 gene locus comprises 12 exons.
  • Alternative splicing of exons 1-5 generates five distinct mB7-2 transcripts with variations in 5' untranslated and signal regions.
  • These transcript variants exhibit differential expression patterns across APC types and are modulated by B cell activation stimuli.

Conclusions:

  • mB7-2 transcripts are subject to differential regulation in a tissue-specific manner.
  • Transcriptional and post-transcriptional regulation contributes to the diverse roles of mB7-2 in immune responses.