Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative cell line based bioassays for human cytokines

A R Mire-Sluis1, L Page, R Thorpe

  • 1Division of Immunobiology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.

Journal of Immunological Methods
|December 1, 1995
PubMed
Summary

Accurate measurement of biologically active cytokines is crucial for research. Cell line bioassays offer precise and reliable methods for quantifying cytokine levels in biological samples.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Accuracy of Carer Measured Weight - A Service Evaluation.

Irish medical journal·2026
Same author

The Association Between Partner Reactions to Chronic Pain and Pain-Related Outcomes: A Systematic Review.

European journal of pain (London, England)·2026
Same author

Validating the Anal Sex Stigma Scale among Black Sexual Minority Men Living with HIV.

Journal of sex research·2025
Same author

The knowledge and attitudes of South African-based runners regarding the use of analgesics during training and competition.

South African journal of sports medicine·2023
Same author

Characteristics associated with antenatally unidentified small-for-gestational-age fetuses: prospective cohort study nested within DESiGN randomized controlled trial.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022
Same author

Improving antenatal detection of small-for-gestational-age fetus: economic evaluation of Growth Assessment Protocol.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Cytokine research is expanding, highlighting their role in diverse physiological and pathological processes.
  • Accurate cytokine measurement is essential for validating research findings.
  • Biological assays are the only method to directly estimate biologically active cytokines.

Purpose of the Study:

  • To review cell line-based bioassays for cytokine measurement.
  • To discuss the advantages and limitations of these bioassays.
  • To identify suitable cell lines for cytokine bioassays.

Main Methods:

  • Review of existing literature on cell line-based cytokine bioassays.
  • Evaluation of criteria for cell line suitability (sensitivity, maintenance, specificity).
  • Discussion of assay design and data analysis considerations.

Related Experiment Videos

Main Results:

  • Cell line-based bioassays are the most accessible, precise, and accurate methods for detecting cytokines.
  • Key criteria for selecting cell lines include sensitivity, ease of maintenance, and specificity.
  • Proper assay design and analysis are critical for valid cytokine level estimation.

Conclusions:

  • Cell line bioassays are a valuable tool for quantifying biologically active cytokines.
  • Selection of appropriate cell lines and assay design are paramount for reliable results.
  • This review provides guidance on utilizing cell line bioassays in cytokine research.