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Related Experiment Videos

Thiols decrease human interleukin (IL) 4 production and IL-4-induced immunoglobulin synthesis

P Jeannin1, Y Delneste, S Lecoanet-Henchoz

  • 1Glaxo Institute for Molecular Biology, Immunology Department, Geneva, Switzerland.

The Journal of Experimental Medicine
|December 1, 1995
PubMed
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N-Acetyl-L-cysteine (NAC) and glutathione (GSH) reduce T-helper 2 cytokine IL-4 production and related immunoglobulin E (IgE) synthesis. These thiol-containing antioxidants selectively inhibit Th2 responses, impacting allergic antibody production in vitro and in vivo.

Area of Science:

  • Immunology
  • Biochemistry
  • Pharmacology

Background:

  • N-Acetyl-L-cysteine (NAC) is an antioxidant and precursor to glutathione (GSH), known for its mucolytic effects.
  • NAC and GSH have demonstrated in vitro effects on T cells, including increased IL-2 production and proliferation.
  • Previous research indicated NAC and GSH modulate T cell functions, but their specific impact on Th2 cytokines and antibody production was less defined.

Purpose of the Study:

  • To investigate the effect of NAC and GSH on Interleukin-4 (IL-4) production by human T cells.
  • To determine the impact of NAC and GSH on IL-4-induced immunoglobulin (Ig) production, particularly IgE.
  • To explore the in vivo effects of NAC on antibody responses.

Main Methods:

  • Stimulated peripheral blood T cells and T helper (Th) clones were treated with NAC and GSH in vitro.

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  • IL-4 production and messenger RNA (mRNA) transcription were measured.
  • Immunoglobulin production by peripheral blood mononuclear cells and purified B cells was assessed.
  • Oral administration of NAC to mice followed by ovalbumin challenge was performed.

    • Main Results:

    • NAC and GSH dose-dependently decreased IL-4 production and IL-4 mRNA transcription in stimulated T cells and Th2 clones.
    • NAC and GSH selectively reduced IL-4-induced IgE and IgG4 production by human peripheral blood mononuclear cells.
    • NAC and GSH decreased mature IgE mRNA in purified B cells, without affecting IgA or IgM production.
    • NAC administration to mice reduced IgE and IgG1 antibody responses to ovalbumin.

    Conclusions:

    • NAC and GSH, along with other thiol-containing molecules, can suppress Th2-derived IL-4 production.
    • These compounds selectively inhibit IL-4-induced immunoglobulin production, particularly IgE.
    • NAC demonstrates potential as an agent to control Th2-mediated allergic responses in vivo.