Related Experiment Videos
Mast cell tryptase levels in normal canine tissues
A D Myles1, R E Halliwell, B Ballauf
1Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
Veterinary Immunology and Immunopathology
|June 1, 1995
Summary
Researchers developed a new enzyme-linked immunosorbent assay (ELISA) to measure canine tryptase in tissues. Canine gut tissue showed the highest tryptase levels, suggesting a significant role for this enzyme in the gut.
Area of Science:
- Veterinary Medicine
- Biochemistry
- Immunology
Background:
- Canine tryptase is an enzyme found in mast cells.
- Understanding tryptase distribution is crucial for diagnosing canine diseases.
- Existing methods for tryptase quantification are limited.
Purpose of the Study:
- To develop and validate a competition enzyme-linked immunosorbent assay (ELISA) for quantifying canine tryptase.
- To determine the tissue distribution of canine tryptase.
- To assess the potential role of this assay in veterinary diagnostics.
Main Methods:
- A competition enzyme-linked immunosorbent assay (ELISA) was developed using rabbit anti-tryptase antibodies.
- The assay quantifies tryptase in tissue extracts within a range of 40-5000 ng ml-1.
- Tissues analyzed included skin, gut, liver, and lung from dogs.
Main Results:
- The developed ELISA assay demonstrated reliable quantification of canine tryptase.
- Canine gut tissue exhibited the highest concentration of tryptase per milligram of wet weight compared to other tissues.
- Skin, liver, and lung tissues also contained measurable levels of canine tryptase.
Conclusions:
- The novel ELISA assay is effective for measuring canine tryptase levels in various tissues.
- The high concentration of tryptase in canine gut suggests a potentially significant functional role at this site.
- This assay can be a valuable tool for investigating mast cell involvement in canine disease states.