Related Experiment Videos
Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps
G Cardinali1, L Pellegrini, A Martini
1Dipartimento di Biologia Vegetale, University of Perugia, Italy.
Yeast (Chichester, England)
|September 15, 1995
Abstract:
A modified procedure is proposed for chromosomal DNA extraction based on a cell-wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non-Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.