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Updated: Sep 9, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
The transformation of c-jun-overexpressing cells is correlated with IGFS-induced c-jun phosphorylation
S Lagarrigue1, C Heberden, P Martel
1Laboratoire de Nutrition et Sécurité Alimentaire-INRA, Jouy en Josas, France.
Abstract:
We have analyzed, in parental and c-jun-transfected rat liver epithelial (REL) cells, the capacity of the insulin-like growth factors (IGF1 and 2) to stimulate growth under anchorage dependent and independent conditions and the role of the c-jun protein in the response to these factors. Although the increase of the proliferation of cells in monolayer was similar for the two lines, only the c-jun-transfected cells formed colonies in soft agar after IGFs exposure. We showed that IGF1 and 2 did not modify the expression of c-fos or c-jun proteins during short-time exposures but both factors enhanced c-jun phosphorylation which suggests that this phenomenon could be involved in IGFs-regulated gene expression. Moreover, we showed that both the overexpression and the increase of phosphorylation status of c-jun protein were necessary to transform REL cells.
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