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Camel trypanosomosis in Rajasthan, India
K M Pathak1, J K Arora, M Kapoor
1College of Veterinary and Animal Science, Bikaner, Rajasthan, India.
Veterinary Parasitology
|September 1, 1993
Summary
Trypanosome infection in camels was studied using two methods. Enzyme-linked immunosorbent assay (ELISA) detected more infections than Giemsa staining, indicating its greater utility for diagnosing current Trypanosoma evansi cases.
Area of Science:
- Veterinary Parasitology
- Camelid Health
- Infectious Disease Diagnostics
Background:
- Trypanosomiasis is a significant disease affecting camel populations.
- Accurate diagnostic methods are crucial for controlling Trypanosoma evansi infections in camels.
Purpose of the Study:
- To compare the efficacy of two diagnostic techniques for detecting trypanosome infection in camels.
- To evaluate the sensitivity of Giemsa staining versus ELISA for identifying Trypanosoma evansi.
Main Methods:
- Examined blood samples from 240 dromedary camels.
- Utilized wet blood Giemsa stain technique for direct parasite detection.
- Employed double antibody sandwich enzyme-linked immunosorbent assay (ELISA) for antigen detection.
Main Results:
- Giemsa staining identified 7.50% (18/240) of camels as infected.
- ELISA detected Trypanosoma evansi antigen in 31.66% (76/240) of camels.
- ELISA demonstrated a higher detection rate compared to Giemsa staining.
Conclusions:
- The double antibody sandwich ELISA is a more sensitive and useful method for diagnosing current Trypanosoma evansi infections in camels.
- ELISA offers a more comprehensive approach to identifying infected camel populations.