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Updated: Jul 7, 2026
10:32
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
Published on: October 25, 2016
Interleukin 1 beta-induced increase in substance P in rat myenteric plexus
S M Hurst1, A M Stanisz, K A Sharkey
1Intestinal Diseases Research Unit, McMaster University, Hamilton, Ontario, Canada.
Gastroenterology
|December 1, 1993
Summary
Interleukin 1 beta (IL-1 beta) stimulates the synthesis of Substance P (SP) in rat intestine nerves. This finding helps explain how inflammation affects nerve signaling and SP production.
Area of Science:
- Neurogastroenterology
- Immunology
- Molecular Biology
Background:
- Substance P (SP) levels rise in the inflamed intestines of rats infected with Trichinella spiralis.
- The precise mechanism behind this increase remains unclear.
- Interleukin 1 beta (IL-1 beta) is present in the longitudinal muscle-myenteric plexus (LM-MP) in this infection model.
Purpose of the Study:
- To investigate if human recombinant IL-1 beta (hrIL-1 beta) can elevate SP levels.
- To examine SP production in LM-MP preparations from non-infected rats when exposed to hrIL-1 beta.
Main Methods:
- Longitudinal muscle-myenteric plexus (LM-MP) preparations were incubated with hrIL-1 beta.
- Immunoreactive SP (IR-SP) levels were quantified using radioimmunoassay.
- Immunohistochemistry was employed to visualize SP distribution within the LM-MP.
Main Results:
- hrIL-1 beta significantly increased IR-SP in a time- and dose-dependent manner, with peak levels at 6 hours and 10 ng/mL.
- Scorpion venom reduced IR-SP, indicating a specific interaction.
- Immunohistochemistry confirmed increased SP staining within LM-MP nerves.
- The effect of IL-1 beta required protein synthesis, was receptor-mediated, and not due to endotoxin.
Conclusions:
- Human recombinant IL-1 beta directly stimulates the synthesis of Substance P.
- This occurs within the myenteric nerves of the rat intestine.
- IL-1 beta plays a role in regulating SP production in the enteric nervous system.
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