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Related Experiment Videos

Stem cell factor induces mast cell adhesion to fibronectin

J Dastych1, D D Metcalfe

  • 1Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Journal of Immunology (Baltimore, Md. : 1950)
|January 1, 1994
PubMed
Summary

Stem cell factor (SCF) promotes mast cell adhesion to fibronectin. This cytokine, produced by stromal cells, may be key for mast cell attachment to connective tissue in the body.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Stem cell factor (SCF), also known as c-kit ligand, is a cytokine produced by stromal cells.
  • SCF is known to influence mast cell proliferation and differentiation.
  • The role of SCF in mast cell adhesion to connective tissue matrix was not well understood.

Purpose of the Study:

  • To investigate the hypothesis that SCF influences mast cell adhesion to connective tissue matrix.
  • To determine the mechanism by which SCF affects mast cell adhesion.
  • To assess the physiological relevance of SCF-induced mast cell adhesion.

Main Methods:

  • Mast cells (MCP5/L and murine bone marrow-derived cultured mast cells - BMCMC) were stimulated with SCF or PMA.
  • Adhesion assays were performed on fibronectin (FN)-coated surfaces.

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  • Experiments involved varying SCF concentrations, using IL-3, assessing calcium dependence, and employing RGD peptides and genistein.

    • Main Results:

    • SCF significantly promoted mast cell adhesion to fibronectin in a dose-dependent manner.
    • SCF-induced adhesion was calcium-dependent and inhibited by RGD peptides, suggesting integrin involvement.
    • Adhesion was mediated by SCF's interaction with c-kit on mast cells, not direct SCF-FN interaction.

    Conclusions:

    • SCF stimulates mast cell adhesion to fibronectin.
    • SCF-induced mast cell adhesion involves integrin receptors and activation via c-kit.
    • SCF is likely a significant factor in the physiological adhesion of mast cells to connective tissue matrix.