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Retrovirus tests of human leukemia/lymphoma cell lines at DSM
1German Collection of Microorganisms and Cell Cultures (DSM), Braunschweig, Germany.
Leukemia & Lymphoma
|October 1, 1993
Abstract:
Permanently established human cell lines can produce several retroviruses. It is important to routinely test such cell lines for human T cell lymphotropic virus (HTLV) type I and II, and for human immunodeficiency virus (HIV) type 1 and 2 in order to exclude any potential biohazard from cell lines producing human retroviruses. Reverse transcriptase assay, polymerase chain reaction, and dot-blot hybridization of in-vitro amplified DNA with virus-specific probes are used.