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Voltage-activated ionic currents in goldfish pituitary cells

C J Price1, J I Goldberg, J P Chang

  • 1Department of Zoology, University of Alberta, Edmonton, Canada.

General and Comparative Endocrinology
|October 1, 1993
PubMed
Summary

Goldfish pituitary cells exhibit electrical excitability and possess voltage-dependent sodium, potassium, and calcium ion channels, crucial for hormone release regulation.

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Area of Science:

  • Neuroendocrinology
  • Cellular Electrophysiology
  • Ion Channel Physiology

Background:

  • Pharmacological studies indicate goldfish pituitary hormone release relies on extracellular calcium influx via voltage-sensitive calcium channels.
  • Understanding the specific ion channels involved is essential for a comprehensive view of pituitary hormone regulation in fish.

Purpose of the Study:

  • To investigate the role of voltage-dependent ion channels in goldfish anterior pituitary hormone release.
  • To characterize cell excitability and specific voltage-dependent ion currents in dispersed goldfish pituitary cells.

Main Methods:

  • Utilized tight-seal whole-cell recordings for electrophysiological characterization of goldfish pituitary cells.
  • Performed voltage-clamp recordings to identify and isolate voltage-dependent Na+, Ca2+, and K+ currents.

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  • Applied specific channel blockers (tetrodotoxin, tetraethylammonium, 4-aminopyridine, verapamil, nifedipine) and barium as a charge carrier.

    • Main Results:

    • Goldfish pituitary cells demonstrated electrical excitability with a resting membrane potential of -36 mV.
    • Identified voltage-dependent Na+ (tetrodotoxin-sensitive), K+ (tetraethylammonium-sensitive delayed rectifier and 4-aminopyridine-sensitive transient currents), and high-voltage activated L-type Ca2+ currents.
    • These ion currents activate at specific membrane potentials and exhibit distinct kinetic and inactivation properties.

    Conclusions:

    • Goldfish pituitary cells possess the necessary electrophysiological properties, including voltage-sensitive ion channels, to regulate hormone release.
    • The identified ion channels are likely integral components in the stimulus-secretion coupling mechanism of goldfish pituitary hormones.