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Bluetongue virus: production and study of viral antigen for serological diagnosis
G M Arita1, M S Gatti, J G Deak
1Laboratorio de Referência Animal, LARA/Campinas, MARA, São Paulo, Brazil.
Journal of Virological Methods
|October 1, 1993
Abstract:
A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 10(3) and 25 x 10(3) NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.